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Bacterial protein patterning by micro-contact printing of PLL-g-PEG

Title data

Saravia, V. ; Küpcü, Seta ; Nolte, Marc ; Huber, C. ; Pum, Dietmar ; Fery, Andreas ; Sleytr, Uwe B. ; Toca-Herrera, José L.:
Bacterial protein patterning by micro-contact printing of PLL-g-PEG.
In: Journal of Biotechnology. Vol. 130 (June 2007) Issue 3 . - pp. 247-252.
ISSN 1873-4863
DOI: https://doi.org/10.1016/j.jbiotec.2007.04.017

Official URL: Volltext

Abstract in another language

Biomimetic micro-patterned surfaces of three S-layer (fusion) proteins, wild type (SbpA), enhanced green fluorescence protein (SbpA-EGFP) and streptavidin (SbpA-STV), were built by microcontact printing of poly-l-lysine grafted polyethylene glycol (PLL-g-PEG). The functionality of the adsorbed proteins was studied with atomic force microscopy and fluorescence microscopy. Atomic force microscopy (AFM) measurements showed that wild-type SbpA recrystallized on PLL-g-PEG free areas, while fluorescent properties of SbpA-EGFP and the interaction of SbpA-streptavidin heterotetramers with biotin were not affected due to the adsorption on the micro patterned substrates.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Physical Chemistry II - Univ.-Prof. Dr. Andreas Fery
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Physical Chemistry II
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT: No
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 530 Physics
500 Science > 540 Chemistry
Date Deposited: 08 Dec 2014 14:31
Last Modified: 18 Feb 2015 14:18
URI: https://eref.uni-bayreuth.de/id/eprint/4018