Bacterial protein patterning by micro-contact printing of PLL-g-PEG

Titelangaben

Saravia, V. ; Küpcü, Seta ; Nolte, Marc ; Huber, C. ; Pum, Dietmar ; Fery, Andreas ; Sleytr, Uwe B. ; Toca-Herrera, José L.:
Bacterial protein patterning by micro-contact printing of PLL-g-PEG.
In: Journal of Biotechnology. Bd. 130 (2007) Heft 3 . - S. 247-252.
ISSN 1873-4863
DOI: https://doi.org/10.1016/j.jbiotec.2007.04.017

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Abstract

Biomimetic micro-patterned surfaces of three S-layer (fusion) proteins, wild type (SbpA), enhanced green fluorescence protein (SbpA-EGFP) and streptavidin (SbpA-STV), were built by microcontact printing of poly-l-lysine grafted polyethylene glycol (PLL-g-PEG). The functionality of the adsorbed proteins was studied with atomic force microscopy and fluorescence microscopy. Atomic force microscopy (AFM) measurements showed that wild-type SbpA recrystallized on PLL-g-PEG free areas, while fluorescent properties of SbpA-EGFP and the interaction of SbpA-streptavidin heterotetramers with biotin were not affected due to the adsorption on the micro patterned substrates.