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Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1

Title data

Zeitvogel, Fabian ; Burkhardt, Claus J. ; Schroeppel, Birgit ; Schmid, Gregor ; Ingino, Pablo ; Obst, Martin:
Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1.
In: Geomicrobiology Journal. Vol. 34 (2017) Issue 4 . - pp. 317-327.
ISSN 1521-0529
DOI: https://doi.org/10.1080/01490451.2016.1189467

Abstract in another language

Innovative microanalytical methods are valuable tools in geomicrobiology. They often require the use of dried samples, demanding a challenging sample preparation. Since geomicrobiological samples typically have a strongly heterogeneous composition, choosing a preparation method is not straightforward. We therefore compared how different drying methods (critical point drying, hexamethyldisilazane drying, air drying and freeze drying) influence the structure of bacterial cell-mineral aggregates. Each method proved suitable for a specific purpose, but none was able to completely preserve the sample structure. Additional information was obtained on surface alterations by sputter coating and on preservation of extracellular polymeric substances during resin embedding.

Further data

Item Type: Article in a journal
Refereed: No
Additional notes: BAYCEER140411
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg-Professur Experimentelle Biogeochemie > Heisenberg-Professur Experimentelle Biogeochemie - Univ.-Prof. Dr. Martin Obst
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg-Professur Experimentelle Biogeochemie
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 03 Jan 2018 13:03
Last Modified: 03 Jan 2018 13:03
URI: https://eref.uni-bayreuth.de/id/eprint/41122