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Characterization and engineering of photoactivated adenylyl cyclases

Titelangaben

Stüven, Birthe ; Stabel, Robert ; Ohlendorf, Robert ; Beck, Julian ; Schubert, Roman ; Möglich, Andreas:
Characterization and engineering of photoactivated adenylyl cyclases.
In: Biological Chemistry. Bd. Online first (9 Januar 2019) .
ISSN 1437-4315
DOI: https://doi.org/10.1515/hsz-2018-0375

Angaben zu Projekten

Projektfinanzierung: Alexander von Humboldt-Stiftung
Deutsche Forschungsgemeinschaft

Abstract

Cyclic nucleoside monophosphates (cNMP) serve as universal second messengers in signal transduction across prokaryotes and eukaryotes. As signaling often relies on transiently formed microdomains of elevated second messenger concentration, means to precisely perturb the spatiotemporal dynamics of cNMPs are uniquely poised for the interrogation of the underlying physiological processes. Optogenetics appears particularly suited as it affords light-dependent, accurate control in time and space of diverse cellular processes. Several sensory photoreceptors function as photoactivated adenylyl cyclases (PAC) and hence serve as light-regulated actuators for the control of intracellular levels of 3′, 5′-cyclic adenosine monophosphate. To characterize PACs and to refine their properties, we devised a test bed for the facile analysis of these photoreceptors. Cyclase activity is monitored in bacterial cells via expression of a fluorescent reporter, and programmable illumination allows the rapid exploration of multiple lighting regimes. We thus probed two PACs responding to blue and red light, respectively, and observed significant dark activity for both. We next engineered derivatives of the red-light-sensitive PAC with altered responses to light, with one variant, denoted DdPAC, showing enhanced response to light. These PAC variants stand to enrich the optogenetic toolkit and thus facilitate the detailed analysis of cNMP metabolism and signaling.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: adenylyl cyclase; BLUF; optogenetics; phytochrome; sensory photoreceptor; synthetic biology
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie > Lehrstuhl Biochemie - Univ.-Prof. Dr. Andreas Möglich
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 15 Jan 2019 14:56
Letzte Änderung: 15 Jan 2019 14:56
URI: https://eref.uni-bayreuth.de/id/eprint/46923