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Requirements for Minus-Strand Transfer Catalyzed by Rous Sarcoma Virus Reverse Transcriptase

Title data

Werner, Susanne ; Vogel-Bachmayr, Karin ; Hollinderbäumer, Britta ; Wöhrl, Birgitta:
Requirements for Minus-Strand Transfer Catalyzed by Rous Sarcoma Virus Reverse Transcriptase.
In: Journal of Virology. Vol. 75 (2001) Issue 21 . - pp. 10132-10138.
ISSN 0022-538X
DOI: https://doi.org/10.1128/JVI.75.21.10132-10138.2001

Abstract in another language

We have examined the specific minus-strand transfer reactions that occur after the synthesis of minus strong-stop DNA and nonspecific strand switching on homopolymeric poly(rA) templates with different types of Rous sarcoma virus (RSV) reverse transcriptases. Three different types of reverse transcriptases can be isolated from virions of RSV: heterodimeric αβ and homodimeric α and β. The mechanism of minus-strand transfer was examined using a model primer-template substrate corresponding to the 5′- and 3′-terminal RNA regions of the RSV genome. The results reveal that the RNase H activity of RSV reverse transcriptases is required for minus-strand transfer. Less than 2% of strand transfer of the extended product is detectable with RNase H-deficient enzymes. We could show that the α homodimer lacking the integrase domain can perform strand transfer almost as efficiently as the αβ and αPol heterodimers. In contrast, the activities of β and Pol for minus-strand transfer are reduced. Furthermore, a two- to fivefold increase in minus-strand transfer activities was observed in the presence of human immunodeficiency virus type 1 nucleocapsid protein.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 17 May 2019 07:16
Last Modified: 17 May 2019 07:16
URI: https://eref.uni-bayreuth.de/id/eprint/48973