Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Homodimeric reverse transcriptases from Rous Sarcoma Virus mutated within the polymerase or RNase H active site of one subunit are active

Title data

Werner, Susanne ; Wöhrl, Birgitta:
Homodimeric reverse transcriptases from Rous Sarcoma Virus mutated within the polymerase or RNase H active site of one subunit are active.
In: European Journal of Biochemistry. Vol. 267 (2000) Issue 15 . - pp. 4740-4744.
ISSN 1432-1033
DOI: https://doi.org/10.1046/j.1432-1327.2000.01530.x

Abstract in another language

Heterodimeric reverse transcriptase (RT) αβ from Rous sarcoma virus (RSV) possesses an asymmetric subunit organization with the polymerase and RNase H active sites localized in the α subunit. To determine whether homodimeric RSV RTs α (63 kDa) or β (95 kDa) assume α subunit organization similar to that of the heterodimer, an essential aspartic acid residue was mutated in the active site of either the polymerase (Asp181 > Asn) or the RNase H (Asp505 > Asn). Homodimeric α or β RT consisting of one wild‐type and one mutated subunit exhibit polymerase or RNase H activity, respectively, whereas the corresponding doubly mutated enzymes are inactive, indicating that the catalytic sites of the polymerase and RNase H domains are formed by only one subunit of the homodimer.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 17 May 2019 07:28
Last Modified: 17 May 2019 07:28
URI: https://eref.uni-bayreuth.de/id/eprint/48975