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Footprint analysis of replicating murine leukemia virus reverse transcriptase

Title data

Wöhrl, Birgitta M ; Georgiadis, MM ; Telesnitsky, A ; Hendrickson, WA ; Le Grice, SFJ:
Footprint analysis of replicating murine leukemia virus reverse transcriptase.
In: Science. Vol. 267 (1995) Issue 5194 . - pp. 96-99.
ISSN 1095-9203
DOI: https://doi.org/10.1126/science.7528942

Abstract in another language

Replication complexes that contained either murine leukemia virus reverse transcriptase (MLV RT) or a variant reverse transcriptase without a ribonuclease (RNase) H domain (delta RH MLV RT) were visualized by enzymatic footprinting. Wild-type MLV RT protected template nucleotides +6 to -27, and primer nucleotides -1 to -26 of primers that had first been extended by one or four nucleotides. Although it catalyzed DNA synthesis, delta RH MLV RT stably bound template-primer only under conditions of reduced ionic strength and protected the duplex portion only as far as position -15. Despite altered hydrolysis profiles, both enzymes covered primarily the template-primer duplex, contradicting recent predictions based on the structure of rat DNA polymerase beta.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 20 May 2019 07:12
Last Modified: 20 May 2019 07:12
URI: https://eref.uni-bayreuth.de/id/eprint/49000