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Cloning and physical mapping of the sor genes for L‐sorbose transport and metabolism from Klebsiella pneumoniae

Title data

Wöhrl, Birgitta M. ; Lengeler, J.W.:
Cloning and physical mapping of the sor genes for L‐sorbose transport and metabolism from Klebsiella pneumoniae.
In: Molecular microbiology. Vol. 4 (1990) Issue 9 . - pp. 1557-1565.
ISSN 1365-2958
DOI: https://doi.org/10.1111/j.1365-2958.1990.tb02067.x

Abstract in another language

The sor genes of Klebsiella pneumoniae KAY2026, which enable the bacterium to metabolize the ketose L‐sorbose, have been cloned on an 8.3kb DNA fragment into the multicopy plasmid, pACYC184. The genes were mapped by restriction analysis, by deletion mapping and by insertion mutagenesis with Tn1725. The corresponding gene products were identified by the maxicell technique. The structural genes sorD, sorA and sorE code for a D‐glucitol‐6‐P dehydrogenase (27 kilodalton (kD)), an Enzymell (EIISor) activity specific for L‐sorbose and an L‐sorbose‐1‐P reductase (45kD). Besides these genes for known functions, three additional genes were discovered: sorC, coding for a transcriptional 40kD regulatory protein, and sorF and sorB, coding for two proteins of 14kD and 19kD, respectively, involved in transport. The genes form an operon (gene order sorCpCDFBAE) and are inducible by L‐sorbose.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Faculties
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 20 May 2019 12:46
Last Modified: 20 May 2019 12:46
URI: https://eref.uni-bayreuth.de/id/eprint/49012