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Wöhrl, Birgitta M. ; Sprenger, Georg A. ; Lengeler, Joseph W.:
Construction of a new catabolic pathway for D-fructose in Escherichia coli K12 using an L-sorbose-specific enzyme from Klebsiella pneumoniae.
In: Archives of Microbiology.
Bd. 154
(1990)
Heft 2
.
- S. 162-167.
ISSN 1432-072X
DOI: https://doi.org/10.1007/BF00423327
Abstract
Starting with a fruK (formerly fpk) mutant of Escherichia coli K12 lacking D-fructose-1-phosphate kinase (E.C. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sorE from Klebsiella pneumoniae coding for an L-sorbose-1-phosphate reductase. The new pathway was shwon to proceed from D-fructose via D-fructose-1-phosphate and D-mannitol-1-phosphate to D-fructose 6-phosphate. It involves a transport system and enzymes encoded in the fru and the mtl operons from E. coli K12 as well as in the sor operon from K. pneumoniae respectively.