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Planarians recruit piRNAs for mRNA turnover in adult stem cells

Title data

Kim, Iana ; Duncan, Elizabeth ; Ross, Eric ; Gorbovytska, Vladyslava ; Nowotarski, Stephanie ; Elliott, Sarah ; Sánchez Alvarado, Alejandro ; Kuhn, Claus-D.:
Planarians recruit piRNAs for mRNA turnover in adult stem cells.
In: Genes & Development. Vol. 33 (November 2019) Issue 21/22 . - pp. 1575-1590.
ISSN 1549-5477
DOI: https://doi.org/10.1101/gad.322776.118

Project information

Project financing: Bayerisches Staatsministerium für Wissenschaft, Forschung und Kunst
Elitenetzwerk Bayern

Abstract in another language

PIWI proteins utilize small RNAs called piRNAs to silence transposable elements, thereby protecting germline integrity. In planarian flatworms, PIWI proteins are essential for regeneration, which requires adult stem cells termed neoblasts. Here, we characterize planarian piRNAs and examine the roles of PIWI proteins in neoblast biology. We find that the planarian PIWI proteins SMEDWI-2 and SMEDWI-3 cooperate to degrade active transposons via the ping-pong cycle. Unexpectedly, we discover that SMEDWI-3 plays an additional role in planarian mRNA surveillance. While SMEDWI-3 degrades numerous neoblast mRNAs in a homotypic ping-pong cycle, it is also guided to another subset of neoblast mRNAs by antisense piRNAs and binds these without degrading them. Mechanistically, the distinct activities of SMEDWI-3 are primarily dictated by the degree of complementarity between target mRNAs and antisense piRNAs. Thus, PIWI proteins enable planarians to repurpose piRNAs for potentially critical roles in neoblast mRNA turnover.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Profile Fields > Advanced Fields > Molecular Biosciences
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Profile Fields
Profile Fields > Advanced Fields
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 11 Sep 2019 09:10
Last Modified: 18 Nov 2019 09:01
URI: https://eref.uni-bayreuth.de/id/eprint/52185