Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

The search for candidate genes associated with natural variation of grain Zn accumulation in barley

Title data

Detterbeck, Amelie ; Nagel, Manuela ; Rensch, Stefan ; Weber, Michael ; Börner, Andreas ; Persson, Daniel P. ; Schjoerring, Jan Kofod ; Christov, Vesselin ; Clemens, Stephan:
The search for candidate genes associated with natural variation of grain Zn accumulation in barley.
In: Biochemical Journal. Vol. 476 (2 July 2019) Issue 13 . - pp. 1889-1909.
ISSN 1470-8728
DOI: https://doi.org/10.1042/BCJ20190181

Abstract in another language

Combating hidden hunger through molecular breeding of nutritionally enriched crops requires a better understanding of micronutrient accumulation. We studied natural variation in grain micronutrient accumulation in barley ( L.) and searched for candidate genes by assessing marker-trait associations (MTAs) and by analyzing transcriptional differences between low and high zinc (Zn) accumulating cultivars during grain filling. A collection of 180 barley lines was grown in three different environments. Our results show a pronounced variation in Zn accumulation, which was under strong genotype influence across different environments. Genome-wide association mapping revealed 13 shared MTAs. Across three environments, the most significantly associated marker was on chromosome 2H at 82.8 cM and in close vicinity to two yellow stripe like (YSL) genes. A subset of two pairs of lines with contrasting Zn accumulation was chosen for detailed analysis. Whole ears and flag leaves were analyzed 15 days after pollination to detect transcriptional differences associated with elevated Zn concentrations in the grain. A putative α-amylase/trypsin inhibitor CMb precursor was decidedly higher expressed in high Zn cultivars in whole ears in all comparisons. Additionally, a gene similar to barley () was found to be a potential candidate gene.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: barley; genome-wide association mapping; grain; natural variation; transcript analysis; zinc
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 570 Life sciences, biology
500 Science > 580 Plants (Botany)
Date Deposited: 25 Sep 2019 07:39
Last Modified: 25 Sep 2019 07:39
URI: https://eref.uni-bayreuth.de/id/eprint/52383