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Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors

Title data

Golonka, David ; Fischbach, Patrick ; Jena, Siddhartha G. ; Kleeberg, Julius R. W. ; Essen, Lars-Oliver ; Toettcher, Jared E. ; Zurbriggen, Matias D. ; Möglich, Andreas:
Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors.
In: Communications Biology. Vol. 2 (2019) . - Art.Nr. 448.
ISSN 2399-3642
DOI: https://doi.org/10.1038/s42003-019-0687-9

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Phytochrome photoreceptors mediate adaptive responses of plants to red and far-red light. These responses generally entail light-regulated association between phytochromes and other proteins, among them the phytochrome-interacting factors (PIF). The interaction with Arabidopsis thaliana phytochrome B (AtPhyB) localizes to the bipartite APB motif of the A. thaliana PIFs (AtPIF). To address a dearth of quantitative interaction data, we construct and analyze numerous AtPIF3/6 variants. Red-light-activated binding is predominantly mediated by the APB N-terminus, whereas the C-terminus modulates binding and underlies the differential affinity of AtPIF3 and AtPIF6. We identify AtPIF variants of reduced size, monomeric or homodimeric state, and with AtPhyB affinities between 10 and 700 nM. Optogenetically deployed in mammalian cells, the AtPIF variants drive light-regulated gene expression and membrane recruitment, in certain cases reducing basal activity and enhancing regulatory response. Moreover, our results provide hitherto unavailable quantitative insight into the AtPhyB:AtPIF interaction underpinning vital light-dependent responses in plants.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 04 Dec 2019 08:12
Last Modified: 04 Dec 2019 08:12
URI: https://eref.uni-bayreuth.de/id/eprint/53473