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Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes

Title data

Schittenhelm, Ralf B. ; Heeger, Sebastian ; Althoff, Friederike ; Walter, Anne ; Heidmann, Stefan ; Mechtler, Karl ; Lehner, Christian F.:
Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes.
In: Chromosoma. Vol. 116 (August 2007) Issue 4 . - pp. 385-402.
ISSN 1432-0886
DOI: https://doi.org/10.1007/s00412-007-0103-y

Abstract in another language

Chromosome segregation during meiosis and mitosis depends on the assembly of functional kinetochores within centromeric regions. Centromeric DNA and kinetochore proteins show surprisingly little sequence conservation despite their fundamental biological role. However, our identification in Drosophila melanogaster of the most diverged orthologs identified so far, which encode components of a kinetochore protein network including the Ndc80 and Mis complexes, further emphasizes the notion of a shared eukaryotic kinetochore design. To determine its spatial organization, we have analyzed by quantitative light microscopy hundreds of native chromosomes from transgenic Drosophila strains coexpressing combinations of red and green fluorescent fusion proteins, fully capable of providing the essential wild-type functions. Thereby, Cenp/A/Cid, Cenp-C, Mis12 and the Ndc80 complex were mapped along the inter sister kinetochore axis with a resolution below 10 nm. The C terminus of Cenp-C was found to be near but well separated from the innermost component Cenp-A/Cid. The N terminus of Cenp-C is further out, clustered with Mis12 and the Spc25 end of the rod-like Ndc80 complex, which is known to bind to microtubules at its other more distal Ndc80/Nuf2 end.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Green Fluorescent Protein; Budding Yeast Kinetochore; Cenp-A; Outer Kinetochore; Centromeric Chromatin; Somatic-Cells; Saccharomyces-Cerevisiae; Caenorhabditis-Eelegans; Crystal-Srtucture; NDC80 Complex
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 05 Mar 2020 10:39
Last Modified: 05 Mar 2020 10:39
URI: https://eref.uni-bayreuth.de/id/eprint/54471