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Schittenhelm, Ralf B. ; Heeger, Sebastian ; Althoff, Friederike ; Walter, Anne ; Heidmann, Stefan ; Mechtler, Karl ; Lehner, Christian F.:
Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes.
In: Chromosoma.
Bd. 116
(2007)
Heft 4
.
- S. 385-402.
ISSN 1432-0886
DOI: https://doi.org/10.1007/s00412-007-0103-y
Abstract
Chromosome segregation during meiosis and mitosis depends on the assembly of functional kinetochores within centromeric regions. Centromeric DNA and kinetochore proteins show surprisingly little sequence conservation despite their fundamental biological role. However, our identification in Drosophila melanogaster of the most diverged orthologs identified so far, which encode components of a kinetochore protein network including the Ndc80 and Mis complexes, further emphasizes the notion of a shared eukaryotic kinetochore design. To determine its spatial organization, we have analyzed by quantitative light microscopy hundreds of native chromosomes from transgenic Drosophila strains coexpressing combinations of red and green fluorescent fusion proteins, fully capable of providing the essential wild-type functions. Thereby, Cenp/A/Cid, Cenp-C, Mis12 and the Ndc80 complex were mapped along the inter sister kinetochore axis with a resolution below 10 nm. The C terminus of Cenp-C was found to be near but well separated from the innermost component Cenp-A/Cid. The N terminus of Cenp-C is further out, clustered with Mis12 and the Spc25 end of the rod-like Ndc80 complex, which is known to bind to microtubules at its other more distal Ndc80/Nuf2 end.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | Green Fluorescent Protein; Budding Yeast Kinetochore; Cenp-A; Outer Kinetochore; Centromeric Chromatin; Somatic-Cells; Saccharomyces-Cerevisiae; Caenorhabditis-Eelegans; Crystal-Srtucture; NDC80 Complex |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Genetik Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 05 Mär 2020 10:39 |
| Letzte Änderung: | 26 Okt 2022 12:35 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/54471 |