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A Novel Isoallergen Dau c 1.0401 in Carrot : Stability, Allergenicity, and Comparison with Other Isoallergens

Title data

Jacob, Thessa ; Wangorsch, Andrea ; Vogel, Lothar ; Reuter, Andreas ; Mahler, Vera ; Wöhrl, Birgitta M.:
A Novel Isoallergen Dau c 1.0401 in Carrot : Stability, Allergenicity, and Comparison with Other Isoallergens.
Biochemie IV ‐ Biopolymere, Universität Bayreuth, Universitätsstr. 30, Bayreuth, D‐95447 Germany; Molecular Allergology, Paul‐Ehrlich‐Institut, Langen, D‐63225 Germany; Division of Allergology, Paul‐Ehrlich‐Institut, Langen, D‐63225 Germany
In: Molecular Nutrition & Food Research. Vol. 65 (2021) Issue 7 . - No. 2001110.
ISSN 1613-4133
DOI: https://doi.org/10.1002/mnfr.202001110

Official URL: Volltext

Abstract in another language

Scope:
Around 25% of food allergic persons in Central Europe suffer from carrot allergy caused by the major carrot allergen Dau c 1. Three different isoallergens, Dau c 1.01, Dau c 1.02 and Dau c 1.03 are identified. However, information about the qualitative and quantitative composition of natural (n)Dau c 1 is scarce.
Methods and Results:
The new carrot allergen Dau c 1.0401 is identified on the mRNA and protein level by RT‐PCR and mass spectrometry. It displays only around 60% sequence identity to the other known Dau c 1 isoallergens. NMR and CD‐spectra are typical for a well‐folded protein containing both α‐helices and β‐strands. It showed a poor refolding capacity after incubation at 95 °C. IgE‐binding is impaired in immunoblots, whereas in inhibition assays IgE binding to soluble Dau c 1.0401 is detected and it clearly provoked a response in mediator release assays.
Conclusion:
Dau c 1.0401 is a new isoallergen which contributes to the allergenicity of carrots. The absence of immunoreactivity in immobilized assays indicates that IgE binding is impaired when the protein is blotted on a solid phase. Altogether, the results point out that its allergenicity can be reduced upon carrot processing.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Allergenicity; Dau c 1 isoallergen; Mass spectrometry; Protein stability; RT‐PCR
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 05 Mar 2021 08:06
Last Modified: 02 Sep 2021 07:12
URI: https://eref.uni-bayreuth.de/id/eprint/63708