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Montenegro, Jose-Maria ; Grazu, Valeria ; Sukhanova, Alyona ; Agarwal, Seema ; de la Fuente, Jesus M. ; Nabiev, Igor ; Greiner, Andreas ; Parak, Wolfgang J.:
Controlled antibody/(bio-) conjugation of inorganic nanoparticles for targeted delivery.
In: Advanced Drug Delivery Reviews.
Bd. 65
(2013)
Heft 5
.
- S. 677-688.
ISSN 0169-409X
DOI: https://doi.org/10.1016/j.addr.2012.12.003
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Angaben zu Projekten
| Projektfinanzierung: |
European Comission, Grant-Number NMP-2009-4.0-3-246479; ERC; MEGA-grant of the Ministry of High Education and Science of the Russian Federation, Grant-Number 11.G3431.0050 |
|---|
Abstract
Abstract Arguably targeting is one of the biggest problems for controlled drug delivery. In the case that drugs can be directed with high efficiency to the target tissue, side effects of medication are drastically reduced. Colloidal inorganic nanoparticles (NPs) have been proposed and described in the last 10 years as new platforms for in vivo delivery. However, though {NPs} can introduce plentiful functional properties (such as controlled destruction of tissue by local heating or local generation of free radicals), targeting remains an issue of intense research efforts. While passive targeting of {NPs} has been reported (the so-called enhanced permeation and retention, {EPR} effect), still improved active targeting would be highly desirable. One classical approach for active targeting is mediated by molecular recognition via capture molecules, i.e. antibodies (Abs) specific for the target. In order to apply this strategy for NPs, they need to be conjugated with Abs against specific biomarkers. Though many approaches have been reported in this direction, the controlled bioconjugation of {NPs} is still a challenge. In this article the strategies of controlled bioconjugation of {NPs} will be reviewed giving particular emphasis to the following questions: 1) how can the number of capture molecules per {NP} be precisely adjusted, and 2) how can the Abs be attached to {NP} surfaces in an oriented way. Solution of both questions is a cornerstone in controlled targeting of the inorganic {NPs} bioconjugates. (C) 2013 Elsevier B.V. All rights reserved.