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Design and Biophysical Characterization of Bioresponsive Degradable Poly(dimethylaminoethyl methacrylate) Based Polymers for In Vitro DNA Transfection

Title data

Zhang, Yi ; Zheng, Mengyao ; Kissel, Thomas ; Agarwal, Seema:
Design and Biophysical Characterization of Bioresponsive Degradable Poly(dimethylaminoethyl methacrylate) Based Polymers for In Vitro DNA Transfection.
In: Biomacromolecules. Vol. 13 (2012) Issue 2 . - pp. 313-322.
ISSN 1526-4602
DOI: https://doi.org/10.1021/bm2015174

Official URL: Volltext

Abstract in another language

Water-soluble, degradable polymers based on poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) with low cytotoxicity and good p-DNA transfection efficiency are highlighted in this article. To solve the nondegradability issue of PDMAEMA, new polymers based on DMAEMA and 5,6-benzo-2-methylene-1,3-dioxepane (BMDO) for gene transfection were synthesized. A poly(ethylene oxide) (PEO) azo-initiator was used as free-radical initiator. PEGylation was performed to improve water solubility and to reduce cytotoxicity of the polymers. The resulting polymers contain hydrolyzable ester linkages in the backbone and were soluble in water even with very high amounts of ester linkages. These degradable copolymers showed significantly less toxicity with a MTT assay using L929 cell lines and demonstrated promising DNA transfection efficiency when compared with the gold standard poly(ethyleneimine). Bioresponsive properties of the corresponding quaternized DMAEMA based degradable polymers were also studied. Although the quaternized DMAEMA copolymers showed enhanced water solubility, they were inferior in gene transfection and toxicity as compared to the unquaternized copolymers.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: PMID: 22191470
Keywords: GENE-DELIVERY; STRUCTURAL-CHARACTERIZATION; COPOLYMERS; THERAPY; COMPLEXES; GLYCOL); 5,6-BENZO-2-METHYLENE-1,3-DIOXEPANE; NANOPARTICLES; CYTOTOXICITY; POLYCATIONS
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II > Chair Macromolecular Chemistry II - Univ.-Prof. Dr. Andreas Greiner
Profile Fields > Advanced Fields > Polymer and Colloid Science
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Profile Fields
Profile Fields > Advanced Fields
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 01 Apr 2015 12:58
Last Modified: 01 Apr 2015 12:58
URI: https://eref.uni-bayreuth.de/id/eprint/9793