at Google ScholarTitle data
Uraguchi, Shimpei ; Kamiya, Takehiro ; Clemens, Stephan ; Fujiwara, Toru:
Characterization of OsLCT1, a cadmium transporter from indica rice (Oryza sativa).
In: Physiologia Plantarum.
Vol. 151
(2014)
Issue 3
.
- pp. 339-347.
ISSN 1399-3054
DOI: https://doi.org/10.1111/ppl.12189
Abstract in another language
Molecular understanding of cadmium (Cd) transport in indica rice (Oryza sativa) is still insufficient, although indica rice generally has a potential to accumulate higher Cd in shoots and grains than japonica rice. We have previously demonstrated that OsLCT1 is a Cd transporter gene responsible for grain Cd accumulation in the japonica model cultivar Nipponbare. In this study, we isolated OsLCT1 cDNA from Kasalath, a model indica (aus subgroup) cultivar and conducted cation transport activity assays in yeast and mRNA expression analysis in plants. The deduced amino acid sequence of Kasalath OsLCT1 is 91.2% identical and 93.8% similar to that of Nipponbare OsLCT1. We established the yeast heterologous system expressing the Kasalath allele of OsLCT1. Elemental profiling of the yeast cells suggested an efflux activity of Kasalath OsLCT1 for Cd, K, Mg, Ca and Mn, but not for Fe, Zn, Cu and Na. This substrate specificity was identical to that of the Nipponbare version. Quantitative RT-PCR showed that expression of OsLCT1 in Kasalath was higher in reproductive stage than in vegetative stage. The expression level of OsLCT1 was significantly higher in Kasalath than in Nipponbare. Phylogenetic analysis found several LCT1-like genes only in grass plants. OsLCT1 is the sole copy in the rice genome and is conserved among each rice subgroup. These newly found LCT homologs will provide a basis for further understanding of LCT-mediated Cd transport.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | BAYCEER121568 |
| Institutions of the University: | Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens Research Institutions Research Institutions > Research Centres Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science 500 Science > 570 Life sciences, biology 500 Science > 580 Plants (Botany) |
| Date Deposited: | 24 Apr 2015 11:56 |
| Last Modified: | 31 Aug 2022 08:52 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/11376 |