at Google ScholarTitle data
Kalusche, Bernhard ; Verscht, Jutta ; Gebauer, Gerhard ; Komor, Ewald ; Haase, Axel:
Sucrose unloading in the hypocotyl of the Ricinus communis L. seedling measured by 13C-nuclear magnetic resonance spectroscopy in vivo.
In: Planta.
Vol. 208
(1999)
Issue 3
.
- pp. 358-364.
ISSN 0032-0935
DOI: https://doi.org/10.1007/s004250050570
Abstract in another language
The unloading of sucrose in the apical part of the hypocotyl of Ricinus communis L. seedlings was measured by 13C-nuclear magnetic resonance (NMR) spectroscopy. The cotyledons of the seedling were immersed in 5 mM Mes buffer containing 100 mM 13C-labeled sucrose. At intervals of 70-90 min, (C-NMR)-13C spectra with broadband decoupling and nuclear Overhauser enhancement were acquired in vivo. The spectra showed growing 13C-resonances of the labeled positions in the sucrose molecule reaching steady-state labeling within 7-8 h. The specific 13C labeling of sucrose in the G(1)-position changed from 0.38 in the supplied sucrose solution to 0.16 in the sucrose extracted from the hypocotyl piece at the end of the experiment (13 h). Labeling of starch (and other insolubles) in the hypocotyl piece was ca. 0.10. It is proposed that the decreased specific labeling of unloaded sucrose is mostly due to the separate local pools of sucrose in the cortex and pith parenchyma, respectively, and less to continuous starch degradation and conversion to sucrose. The report gives an example of the application of 13C-NMR spectroscopy in assimilate allocation studies.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | BAYCEER22430 |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Ecology Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Research Institutions Research Institutions > Research Centres |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science |
| Date Deposited: | 24 Sep 2015 09:38 |
| Last Modified: | 24 Sep 2015 09:38 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19713 |