Title data
Sakuth, Thomas ; Schobert, Christian ; Pecsvaradi, Attila ; Eichholz, Arnulf ; Komor, Ewald ; Orlich, Gabriele:
Specific proteins in the sieve-tube exudate of ricinus-communis l seedlings - separation, characterization and in-vivo labeling.
In: Planta.
Vol. 191
(1993)
Issue 2
.
- pp. 207-213.
ISSN 0032-0935
DOI: https://doi.org/10.1007/BF00199751
Abstract in another language
Ricinus communis L. seedlings exuded pure phloem sap from the cut hypocotyl for several hours. Throughout the entire exudation period proteins were present in the phloem exudate at a constant concentration ranging from 0.11 to 0.41 mg.ml-1 depending on the culture conditions and the age of the seedlings. Manipulation of the nutrient supply at the cotyledons after removal of the endosperm did not change the protein concentration in the exudate. Comparison of sieve-tube exudate proteins (STEPs) with soluble proteins extracted from the hypocotyl and the cotyledons showed a unique abundance of small proteins in the exudate, with molecular weights ranging from 10 to 25 kDa. Bands at 18, 19 and 20 kDa were especially dominant. The proteins found transiently in the xylem exudate, which might represent proteins secreted at the wound surface, were different in pattern. Two-dimensional separation of STEPs revealed that more than 100 distinct polypeptides occurred in the sieve-tube exudate, most of them slightly acidic with isoelectric points ranging from 4 to 6 and a few basic ones around 8. [S-35]Methionine fed to the cotyledons led to labelling of STEPs, demonstrating their rapid synthesis. It is concluded that there is a continuous synthesis and translocation of specific sieve-tube proteins, whose function is unknown.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Additional notes: | BAYCEER22535 |
Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Research Institutions Research Institutions > Research Centres |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science |
Date Deposited: | 05 Feb 2016 07:23 |
Last Modified: | 05 Feb 2016 07:23 |
URI: | https://eref.uni-bayreuth.de/id/eprint/30436 |