Library-Aided Probing of Linker Determinants in Hybrid Photoreceptors

Title data

Ohlendorf, Robert ; Schumacher, Charlotte Helene ; Richter, Florian ; Möglich, Andreas:
Library-Aided Probing of Linker Determinants in Hybrid Photoreceptors.
In: ACS Synthetic Biology. Vol. 5 (2016) Issue 10 . - pp. 1117-1126.
ISSN 2161-5063
DOI: https://doi.org/10.1021/acssynbio.6b00028

Official URL:

Abstract in another language

Signaling proteins comprise interaction and effector modules connected by linkers. Throughout evolution, these recurring modules have multiply been recombined to produce the present-day plethora of signaling proteins. Likewise, modular recombination lends itself to the engineering of hybrid signal receptors, whose functionality hinges on linker topology, sequence, and length. Often, numerous linkers must be assessed to obtain functional receptors. To expedite linker optimization, we devised the PATCHY strategy (primer-aided truncation for the creation of hybrid proteins) for the facile construction of hybrid gene libraries with defined linker distributions. Empowered by PATCHY, we engineered photoreceptors whose signal response was governed by linker length: whereas blue-light-repressed variants possessed linkers of 7n or 7n+5 residues, variants with 7n+1 residues were blue-light-activated. Related natural receptors predominantly displayed linker lengths of 7n and 7n+5 residues but rarely of 7n+1 residues. PATCHY efficiently explores linker sequence space to yield functional hybrid proteins including variants transcending the natural repertoire of signaling proteins.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Additional notes:	PubMed-ID: 27002379
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Professorship Biochemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Result of work at the UBT:	No
DDC Subjects:	500 Science > 540 Chemistry
Date Deposited:	21 Sep 2016 06:31
Last Modified:	20 Oct 2023 07:40
URI:	https://eref.uni-bayreuth.de/id/eprint/34753