at Google ScholarTitle data
Richter, Florian ; Fonfara, Ines ; Gelfert, Renate ; Nack, Jennifer ; Charpentier, Emmanuelle ; Möglich, Andreas:
Switchable Cas9.
In: Current Opinion in Biotechnology.
Vol. 48
(2017)
.
- pp. 119-126.
ISSN 1879-0429
DOI: https://doi.org/10.1016/j.copbio.2017.03.025
Abstract in another language
Ever since its discovery, Cas9 from Streptococcus pyogenes has revolutionized biology by enabling analysis and engineering of genomes with unprecedented precision and ease. To fine-tune on-target effects and to mitigate adverse effects caused by untimely and off-target action of Cas9, strategies have been developed to control its activity at the post-translational stage via external trigger signals. Control is either achieved by modifying the Cas9 protein itself or its programmable RNA molecules. To date, switchable Cas9 variants responding to small ligands, light or temperature have been engineered. With these variants in hand, the regulation and modification of genomes can be accomplished in graded and ever more precise manner.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 03 May 2017 08:37 |
| Last Modified: | 25 Apr 2022 06:18 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/36918 |