at Google ScholarTitle data
Berntsson, Oskar ; Diensthuber, Ralph P. ; Panman, Matthijs R. ; Björling, Alexander ; Hughes, Ashley J. ; Henry, Léocadie ; Niebling, Stephan ; Newby, Gemma ; Liebi, Marianne ; Menzel, Andreas ; Henning, Robert ; Kosheleva, Irina ; Möglich, Andreas ; Westenhoff, Sebastian:
Time-Resolved X-Ray Solution Scattering Reveals the Structural Photoactivation of a Light-Oxygen-Voltage Photoreceptor.
In: Structure.
Vol. 25
(2017)
.
- pp. 1-6.
ISSN 0969-2126
DOI: https://doi.org/10.1016/j.str.2017.04.006
Abstract in another language
Light-oxygen-voltage (LOV) receptors are sensory proteins controlling a wide range of organismal adaptations in multiple kingdoms of life. Because of their modular nature, LOV domains are also attractive for use as optogenetic actuators. A flavin chromophore absorbs blue light, forms a bond with a proximal cysteine residue, and induces changes in the surroundings. There is a gap of knowledge on how this initial signal is relayed further through the sensor to the effector module. To characterize these conformational changes, we apply time-resolved X-ray scattering to the homodimeric LOV domain from Bacillus subtilis YtvA. We observe a global structural change in the LOV dimer synchronous with the formation of the chromophore photoproduct state. Using molecular modeling, this change is identified as splaying apart and relative rotation of the two monomers, which leads to an increased separation at the anchoring site of the effector modules.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Keywords: | light-oxygen-voltage; Photocycle; protein structural dynamics; sensor histidine kinase; sensory photoreceptor; Signal Transduction; X-ray solution scattering |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 12 May 2017 06:04 |
| Last Modified: | 12 May 2017 06:04 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/37116 |