at Google ScholarTitle data
Young, Seth L. ; Gupta, Maneesh ; Hanske, Christoph ; Fery, Andreas ; Scheibel, Thomas ; Tsukruk, Vladimir V.:
Utilizing conformational changes for patterning thin films of recombinant spider silk proteins.
In: Biomacromolecules.
Vol. 13
(2012)
Issue 10
.
- pp. 3189-3199.
ISSN 1526-4602
DOI: https://doi.org/10.1021/bm300964h
Abstract in another language
Recombinant spider silk proteins mimicking the properties of dragline silk proteins represent a class of materials that hold great potential for future high-performance applications. Here we explore the self-assembly behavior of a recombinantly produced spider silk protein based on the dragline silk of the Araneus diadematus , eADF4 (C16), by selectively patterning its secondary structure using capillary transfer lithography and solvent-assisted microcontact molding. Two conformational transitions were observed, influenced by initial solvent composition: α-helix/random coil conformation to a more densely packed β-sheet conformation (by casting from 1,1,1,3,3,3-hexafluoro-propanol) and moderate initial β-sheet content to higher β-sheet content (casting from formic acid). Furthermore, by using the solvent-assisted microcontact molding technique, we were able to achieve a submicrometer spatial resolution and reveal fine details of morphological and mechanical changes in patterned regions and at interfaces.