Title data
Kuntz, Sabine ; Kunz, Clemens ; Domann, Eugen ; Würdemann, Nora ; Unger, Franziska ; Römpp, Andreas ; Rudloff, Silvia:
Inhibition of Low-Grade Inflammation by Anthocyanins after Microbial Fermentation in Vitro.
In: Nutrients.
Vol. 8
(2016)
Issue 7
.
- p. 411.
ISSN 2072-6643
DOI: https://doi.org/10.3390/nu8070411
Abstract in another language
The anti-inflammatory effects of anthocyanins (ACNs) on vascular functions are discussed controversially because of their low bioavailability. This study was performed to determine whether microorganism (MO)-fermented ACNs influence vascular inflammation in vitro. Therefore, MO growth media were supplemented with an ACN-rich grape/berry extract and growth responses of Escherichia coli, E. faecalis and H. alvei, as well as ACN fermentation were observed. MO supernatants were used for measuring the anti-inflammatory effect of MO-fermented ACNs in an epithelial-endothelial co-culture transwell system. After basolateral enrichment (240 min), endothelial cells were stimulated immediately or after 20 h with TNF-α. Afterwards, leukocyte adhesion, expression of adhesion molecules and cytokine release were measured. Results indicate that E. coli, E. faecalis and H. alvei utilized ACNs differentially concomitant with different anti-inflammatory effects. Whereas E. coli utilized ACNs completely, no anti-inflammatory effects of fermented ACNs were observed on activated endothelial cells. In contrast, ACN metabolites generated by E. faecalis and H. alvei significantly attenuated low-grade stimulated leukocyte adhesion, the expression of adhesion molecules E-selectin, VCAM-1 and ICAM-1 and cytokine secretion (IL-8 and IL-6), as well as NF-κB mRNA expression with a more pronounced effect of E. faecalis than H. alvei. Thus, MO-fermented ACNs have the potential to reduce inflammation.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Bioanalytical Sciences and Food Analytics > Chair Bioanalytical Sciences and Food Analytics - Univ.-Prof. Dr. Andreas Römpp Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Bioanalytical Sciences and Food Analytics |
Result of work at the UBT: | No |
DDC Subjects: | 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
Date Deposited: | 09 Jul 2018 07:07 |
Last Modified: | 09 Jul 2018 08:20 |
URI: | https://eref.uni-bayreuth.de/id/eprint/44987 |