at Google ScholarTitle data
Rib-Schmidt, Carina ; Riedl, Philipp ; Meisinger, Veronika ; Schwaben, Luisa ; Schulenborg, Thomas ; Reuter, Andreas ; Schiller, Dirk ; Seutter von Loetzen, Christian ; Rösch, Paul:
pH and Heat Resistance of the Major Celery Allergen Api g 1.
In: Molecular Nutrition & Food Research.
Vol. 62
(2018)
Issue 15
.
- 1700886.
ISSN 1613-4133
DOI: https://doi.org/10.1002/mnfr.201700886
Abstract in another language
SCOPE:
The major celery allergen Api g 1 is a member of the pathogenesis-related 10 class protein family. This study aims to investigate the impact of heat and pH on the native protein conformation required for Immunoglobulin E (IgE) recognition.
METHODS AND RESULTS:
Spectroscopic methods, MS and IgE-binding analyses are used to study the effects of pH and thermal treatment on Api g 1.0101. Heat processing results in a loss of the native protein fold via denaturation, oligomerization, and precipitation along with a subsequent reduction of IgE recognition. The induced effects and timescales are strongly pH dependent. While Api g 1 refolds partially into an IgE-binding conformation at physiological pH, acidic pH treatment leads to the formation of structurally heat-resistant, IgE-reactive oligomers. Thermal processing in the presence of a celery matrix or at pH conditions close to the isoelectric point (pI = 4.63) of Api g 1.0101 results in almost instant precipitation.
CONCLUSION:
This study demonstrates that Api g 1.0101 is not intrinsically susceptible to heat treatment in vitro. However, the pH and the celery matrix strongly influence the stability of Api g 1.0101 and might be the main reasons for the observed temperature lability of this important food allergen.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Date Deposited: | 30 Jan 2019 12:16 |
| Last Modified: | 22 Nov 2023 10:28 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/47137 |