Interaction of tRNA(Lys-3) with multiple forms of human immunodeficiency virus reverse transcriptase

Title data

Richter-Cook, Nancy J. ; Howard, Kathryn J. ; Cirino, Nick M. ; Wöhrl, Birgitta M. ; Le Grice, Stuart F.J.:
Interaction of tRNA(Lys-3) with multiple forms of human immunodeficiency virus reverse transcriptase.
In: The Journal of Biological Chemistry. Vol. 267 (1992) Issue 22 . - pp. 15952-15957.
ISSN 1083-351X

Official URL:

Abstract in another language

The interaction of several forms (p51, p66, and p66/p51) of recombinant human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) with a synthetic derivative of its cognate replication primer, tRNALys-3, has been determined by gel-mobility shift analysis. While p66/p51 RT is proficient in tRNA binding, preparations of p66 and p51 display only weak binding at elevated protein:tRNA ratios, despite the former containing both RNA-dependent DNA polymerase and ribonuclease H (RNase H) activity. Gel permeation analysis of purified p66 RT indicate this to be predominantly monomeric, suggesting that dimerization may be a prerequisite for efficient tRNA binding. Prolonged incubation of a mixture of the 66- and 51- kDa polypeptides results in heterodimer reconstitu- tion, restoration of tRNA binding, and recovery of appreciable levels of RNA-dependent DNA polymerase activity. Under the same conditions, both the tRNA binding and RNA dependent DNA polymerase activities of the 66- and 51-kDa polypeptides are unaffected, suggesting that they remain in the monomeric conformation.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Result of work at the UBT:	No
DDC Subjects:	500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology
Date Deposited:	20 May 2019 12:08
Last Modified:	20 May 2019 12:08
URI:	https://eref.uni-bayreuth.de/id/eprint/49007