at Google ScholarTitle data
Wöhrl, Birgitta M. ; Lengeler, Joseph W.:
Cloning and physical mapping of the sor genes for L‐sorbose transport and metabolism from Klebsiella pneumoniae.
In: Molecular Microbiology.
Vol. 4
(1990)
Issue 9
.
- pp. 1557-1565.
ISSN 1365-2958
DOI: https://doi.org/10.1111/j.1365-2958.1990.tb02067.x
Abstract in another language
The sor genes of Klebsiella pneumoniae KAY2026, which enable the bacterium to metabolize the ketose L‐sorbose, have been cloned on an 8.3kb DNA fragment into the multicopy plasmid, pACYC184. The genes were mapped by restriction analysis, by deletion mapping and by insertion mutagenesis with Tn1725. The corresponding gene products were identified by the maxicell technique. The structural genes sorD, sorA and sorE code for a D‐glucitol‐6‐P dehydrogenase (27 kilodalton (kD)), an Enzymell (EIISor) activity specific for L‐sorbose and an L‐sorbose‐1‐P reductase (45kD). Besides these genes for known functions, three additional genes were discovered: sorC, coding for a transcriptional 40kD regulatory protein, and sorF and sorB, coding for two proteins of 14kD and 19kD, respectively, involved in transport. The genes form an operon (gene order sorCpCDFBAE) and are inducible by L‐sorbose.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology |
| Date Deposited: | 20 May 2019 12:46 |
| Last Modified: | 11 Feb 2021 08:56 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/49012 |