at Google ScholarTitle data
Schmid, Gregor ; Zeitvogel, Fabian ; Hao, Likai ; Ingino, Pablo ; Kuerner, Wolfgang ; Dynes, James J. ; Karunakaran, Chithra ; Wang, Jian ; Lu, Yingshen ; Ayers, Travis ; Schietinger, Chuck ; Hitchcock, Adam P. ; Obst, Martin:
Synchrotron-Based Chemical Nano-Tomography of Microbial Cell-Mineral Aggregates in their Natural, Hydrated State.
In: Microscopy and Microanalysis.
Vol. 20
(2014)
Issue 2
.
- pp. 531-536.
ISSN 1435-8115
DOI: https://doi.org/10.1017/S1431927613014104
Abstract in another language
Chemical nano-tomography of microbial cells in their natural, hydrated state provides direct evidence of metabolic and chemical processes. Cells of the nitrate-reducing Acidovorax sp. strain BoFeN1 were cultured in the presence of ferrous iron. Bacterial reduction of nitrate causes precipitation of Fe(III)-(oxyhydr)oxides in the periplasm and in direct vicinity of the cells. Nanoliter aliquots of cell-suspension were injected into custom- designed sample holders wherein polyimide membranes collapse around the cells by capillary forces. The immobilized, hydrated cells were analyzed by synchrotron-based scanning transmission X-ray microscopy in combination with angle-scan tomography. This approach provides three-dimensional (3D) maps of the chemical species in the sample by employing their intrinsic near-edge X-ray absorption properties. The cells were scanned through the focus of a monochromatic soft X-ray beam at different, chemically specific X-ray energies to acquire projection images of their corresponding X-ray absorbance. Based on these images, chemical composition maps were then calculated. Acquiring projections at different tilt angles allowed for 3D reconstruction of the chemical composition. Our approach allows for 3D chemical mapping of hydrated samples and thus provides direct evidence for the localization of metabolic and chemical processes in situ.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | BAYCEER135397 |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences > Heisenberg Professorship - Experimental Biogeochemistry > Heisenberg Professorship - Experimental Biogeochemistry - Univ.-Prof. Dr. Martin Obst Research Institutions Research Institutions > Research Centres Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science |
| Date Deposited: | 13 Aug 2020 08:44 |
| Last Modified: | 09 Mar 2022 13:52 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/56469 |