Title data
Pilsl, Sebastian ; Morgan, Charles ; Choukeife, Moujab ; Möglich, Andreas ; Mayer, Günter:
Optoribogenetic control of regulatory RNA molecules.
In: Nature Communications.
Vol. 11
(2020)
Issue 1
.
- No. 4825.
ISSN 2041-1723
DOI: https://doi.org/10.1038/s41467-020-18673-5
Project information
Project financing: |
Deutsche Forschungsgemeinschaft |
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Abstract in another language
Short regulatory RNA molecules underpin gene expression and govern cellular state and physiology. To establish an alternative layer of control over these processes, we generated chimeric regulatory RNAs that interact reversibly and light-dependently with the light-oxygen-voltage photoreceptor PAL. By harnessing this interaction, the function of micro RNAs (miRs) and short hairpin (sh) RNAs in mammalian cells can be regulated in a spatiotemporally precise manner. The underlying strategy is generic and can be adapted to near-arbitrary target sequences. Owing to full genetic encodability, it establishes optoribogenetic control of cell state and physiology. The method stands to facilitate the non-invasive, reversible and spatiotemporally resolved study of regulatory RNAs and protein function in cellular and organismal environments.
Further data
Item Type: | Article in a journal |
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Refereed: | Yes |
Additional notes: | Number: 1 Publisher: Nature Publishing Group |
Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science > 570 Life sciences, biology |
Date Deposited: | 29 Sep 2020 07:14 |
Last Modified: | 29 Sep 2020 07:14 |
URI: | https://eref.uni-bayreuth.de/id/eprint/57732 |