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Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks

Titelangaben

Mejean, Cecile O. ; Schaefer, Andrew W. ; Buck, Kenneth B. ; Kress, Holger ; Shundrovsky, Alla ; Merrill, Jason W. ; Dufresne, Eric R. ; Forscher, Paul:
Elastic Coupling of Nascent apCAM Adhesions to Flowing Actin Networks.
In: PLoS One. Bd. 8 (2013) Heft 9 . - e73389.
ISSN 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0073389

Volltext

Link zum Volltext (externe URL): Volltext

Abstract

Adhesions are multi-molecular complexes that transmit forces generated by a cell’s acto-myosin networks to external substrates. While the physical properties of some of the individual components of adhesions have been carefully characterized, the mechanics of the coupling between the cytoskeleton and the adhesion site as a whole are just beginning to be revealed. We characterized the mechanics of nascent adhesions mediated by the immunoglobulin-family cell adhesion molecule apCAM, which is known to interact with actin filaments. Using simultaneous visualization of actin flow and quantification of forces transmitted to apCAM-coated beads restrained with an optical trap, we found that adhesions are dynamic structures capable of transmitting a wide range of forces. For forces in the picoNewton scale, the nascent adhesions’ mechanical properties are dominated by an elastic structure which can be reversibly deformed by up to 1 µm. Large reversible deformations rule out an interface between substrate and cytoskeleton that is dominated by a number of stiff molecular springs in parallel, and favor a compliant cross-linked network. Such a compliant structure may increase the lifetime of a nascent adhesion, facilitating signaling and reinforcement.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Professur Experimentalphysik VI - Biologische Physik > Professur Experimentalphysik VI - Biologische Physik - Univ.-Prof. Dr. Holger Kreß
Fakultäten
Fakultäten > Fakultät für Mathematik, Physik und Informatik
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut
Fakultäten > Fakultät für Mathematik, Physik und Informatik > Physikalisches Institut > Professur Experimentalphysik VI - Biologische Physik
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 26 Feb 2021 09:44
Letzte Änderung: 01 Sep 2022 07:40
URI: https://eref.uni-bayreuth.de/id/eprint/63454