Title data
Multamäki, Elina ; García de Funtes, Andrés ; Sieryi, Oleksii ; Bykov, Alexander ; Gerken, Uwe ; Ranzani, Américo Tavares ; Köhler, Jürgen ; Meglinski, Igor ; Möglich, Andreas ; Takala, Heikki:
Optogenetic Control of Bacterial Expression by Red Light.
In: ACS Synthetic Biology.
(August 2022)
.
ISSN 2161-5063
DOI: https://doi.org/10.1021/acssynbio.2c00259
Project information
Project financing: |
Deutsche Forschungsgemeinschaft |
---|
Abstract in another language
In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors. Here, we apply this insight to reprogram two widely used setups for bacterial gene expression from blue-light to red-light control. The resultant pREDusk and pREDawn systems allow gene expression to be regulated down and up, respectively, uniformly under red light by 100-fold or more. Both setups are realized as portable, single plasmids that encode all necessary components including the biliverdin-producing machinery. The triggering by red light affords high spatial resolution down to the single-cell level. As pREDusk and pREDawn respond sensitively to red light, they support multiplexing with optogenetic systems sensitive to other light colors. Owing to the superior tissue penetration of red light, the pREDawn system can be triggered at therapeutically safe light intensities through material layers, replicating the optical properties of the skin and skull. Given these advantages, pREDusk and pREDawn enable red-light-regulated expression for diverse use cases in bacteria.
Further data
Item Type: | Article in a journal |
---|---|
Refereed: | Yes |
Keywords: | two-component system; sensory photoreceptor; signal transduction; gene expression; phytochrome |
Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry |
Result of work at the UBT: | Yes |
DDC Subjects: | 500 Science > 570 Life sciences, biology |
Date Deposited: | 14 Oct 2022 05:41 |
Last Modified: | 14 Oct 2022 05:41 |
URI: | https://eref.uni-bayreuth.de/id/eprint/72426 |