Lab in a Tube : Purification, Amplification, and Detection of DNA Using Poly(2-oxazoline) Multilayers

Title data

Leiske, Meike N. ; Hartlieb, Matthias ; Paulenz, Christian ; Pretzel, David ; Hentschel, Martin ; Englert, Christoph ; Gottschaldt, Michael ; Schubert, Ulrich S.:
Lab in a Tube : Purification, Amplification, and Detection of DNA Using Poly(2-oxazoline) Multilayers.
In: Advanced Functional Materials. Vol. 25 (2015) Issue 16 . - pp. 2458-2466.
ISSN 1616-3028
DOI: https://doi.org/10.1002/adfm.201404510

Official URL:

Abstract in another language

Fast and easy purification and amplification of DNA are prerequisites for the development of point-of-care diagnostics. For this reason covalent coatings of amine containing poly(2-oxazoline)s (POx) on glass and poly(propylene) surfaces are prepared, to reversibly bind genetic material directly from biological samples. The polymer is deposited in a layer-by-layer process, whereas initial immobilization of macromolecules on the surface is accomplished by the use of an epoxy functionalized siloxane monolayer. Alternating treatment with polymer and cross-linker leads to the construction of amine containing POx multilayers on the substrates. Successful deposition is investigated by confocal laser scanning microscopy (using labeled polymers), contact angle measurements, as well as reflectometric interference spectroscopy. The interaction of these layer systems with DNA regarding binding and temperature dependent release is studied using labeled genetic material. Finally, polymerase chain reaction (PCR) vessels are coated with POx layers on the inside, and used for quantitative real-time PCR (qPCR) experiments. It is possible to bind genetic material directly from cell lysates to perform qPCR assays from surface adsorbed DNA within the same tube including amplification, as well as detection. The presented system displays an easy to use device for a point of care diagnostic.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Keywords:	bioanalytics; DNA; lab in a tube; layer-by-layer; poly(2-oxazoline)
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Junior Professor Sustainable and Functional Polymer Systems > Junior Professor Sustainable and Functional Polymer Systems - Juniorprof. Dr. Meike Leiske Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Junior Professor Sustainable and Functional Polymer Systems
Result of work at the UBT:	No
DDC Subjects:	500 Science > 540 Chemistry
Date Deposited:	18 Jan 2023 14:46
Last Modified:	13 Mar 2023 08:44
URI:	https://eref.uni-bayreuth.de/id/eprint/73453