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Lab in a Tube : Purification, Amplification, and Detection of DNA Using Poly(2-oxazoline) Multilayers

Titelangaben

Leiske, Meike N. ; Hartlieb, Matthias ; Paulenz, Christian ; Pretzel, David ; Hentschel, Martin ; Englert, Christoph ; Gottschaldt, Michael ; Schubert, Ulrich S.:
Lab in a Tube : Purification, Amplification, and Detection of DNA Using Poly(2-oxazoline) Multilayers.
In: Advanced Functional Materials. Bd. 25 (2015) Heft 16 . - S. 2458-2466.
ISSN 1616-3028
DOI: https://doi.org/10.1002/adfm.201404510

Volltext

Link zum Volltext (externe URL): Volltext

Abstract

Fast and easy purification and amplification of DNA are prerequisites for the development of point-of-care diagnostics. For this reason covalent coatings of amine containing poly(2-oxazoline)s (POx) on glass and poly(propylene) surfaces are prepared, to reversibly bind genetic material directly from biological samples. The polymer is deposited in a layer-by-layer process, whereas initial immobilization of macromolecules on the surface is accomplished by the use of an epoxy functionalized siloxane monolayer. Alternating treatment with polymer and cross-linker leads to the construction of amine containing POx multilayers on the substrates. Successful deposition is investigated by confocal laser scanning microscopy (using labeled polymers), contact angle measurements, as well as reflectometric interference spectroscopy. The interaction of these layer systems with DNA regarding binding and temperature dependent release is studied using labeled genetic material. Finally, polymerase chain reaction (PCR) vessels are coated with POx layers on the inside, and used for quantitative real-time PCR (qPCR) experiments. It is possible to bind genetic material directly from cell lysates to perform qPCR assays from surface adsorbed DNA within the same tube including amplification, as well as detection. The presented system displays an easy to use device for a point of care diagnostic.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: bioanalytics; DNA; lab in a tube; layer-by-layer; poly(2-oxazoline)
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Juniorprofessur Nachhaltige und funktionale Polymersysteme > Juniorprofessur Nachhaltige und funktionale Polymersysteme - Juniorprof. Dr. Meike Leiske
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Juniorprofessur Nachhaltige und funktionale Polymersysteme
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 540 Chemie
Eingestellt am: 18 Jan 2023 14:46
Letzte Änderung: 13 Mär 2023 08:44
URI: https://eref.uni-bayreuth.de/id/eprint/73453