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Microvascular development in the rat arteriovenous loop model in vivo : A step by step intravital microscopy analysis

Title data

Vaghela, Ravikumar ; Arkudas, Andreas ; Gage, Daniel ; Körner, Carolin ; von Hörsten, Stephan ; Salehi, Sahar ; Horch, Raymund E. ; Hessenauer, Maximilian:
Microvascular development in the rat arteriovenous loop model in vivo : A step by step intravital microscopy analysis.
In: Journal of Biomedical Materials Research Part A. Vol. 110 (2022) Issue 9 . - pp. 1551-1563.
ISSN 1552-4965

Abstract in another language

The arteriovenous (AV) loop model is a key technique to solve one of the major problems of tissue engineering—providing adequate vascular support for a tissue construct of significant size. However, the molecular and cellular mechanisms of vascularization and factors influencing the generation of new tissue in the AV loop are still poorly understood. We previously established a novel intravital microscopy approach to study these events. In this study, we implanted our observation chamber filled with two types of hydrogels such as fibrin and methacrylate gelatin (GelMA) and performed intravital microscopy (IVM) on days 7, 14, and 21. Initial microvessel formation was observed in GelMA on day 14, while the vessel network showed clear indicators of network rearrangement and maturation on day 21. No visible microvessels were observed in fibrin. The chambers were explanted on day 21. Histological examination revealed higher numbers of microvessels in GelMA compared to fibrin, while the AV loop was thrombosed in all fibrin constructs, possibly due to matrix degradation. GelMA proved to be an ideal matrix for IVM studies in the AV loop model due to its slow degradation and transparency. This IVM model can be employed as a novel tool for live and thus faster comprehension of crucial events in the tissue regeneration process, which can improve tissue engineering application.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Biomaterials
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 610 Medicine and health
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 08 Mar 2023 14:12
Last Modified: 08 Mar 2023 14:12