bei Google ScholarTitelangaben
Hessenauer, Maximilian ; Vaghela, Ravikumar ; Körner, Carolin ; von Hörsten, Stephan ; Pobel, Christoph ; Gage, Daniel ; Müller, Claudia ; Salehi, Sahar ; Horch, Raymund E. ; Arkudas, Andreas:
Watching the Vessels Grow : Establishment of Intravital Microscopy in the Arteriovenous Loop Rat Model.
In: Tissue Engineering Part C: Methods.
Bd. 27
(2021)
Heft 6
.
- S. 357-365.
ISSN 2152-4955
DOI: https://doi.org/10.1089/ten.tec.2021.0024
Abstract
Tissue engineering in reconstructive surgery seeks to generate bioartificial tissue substitutes. The arteriovenous (AV) loop allows the generation of axially vascularized tissue constructs. Cellular mechanisms of this vascularization process are largely unclear. In this study, we developed two different chamber models for intravital microscopy and imaging of the AV loop in the rat. Multiple design variations were implanted and the stability of the chamber and AV loop patency was tested in vivo. Our novel chamber facilitates repetitive observation of the AV loop using fluorescence-enhanced intravital microscopy. This technique can be used for daily evaluation of leukocyte-endothelial cell interactions, vascularization, and tissue formation in the AV loop model on 14 consecutive days. Therefore, our newly developed model for intravital microscopy will provide better understanding of cellular and molecular processes in tissue engineering in the AV loop. Moreover, it supports initiation of the novel approaches for therapeutic applications.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 09 Mär 2023 10:23 |
| Letzte Änderung: | 09 Mär 2023 10:23 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/74151 |