Measuring Nuclear Mechanics with Atomic Force Microscopy

Titelangaben

dos Santos, Ália ; Rehfeldt, Florian ; Toseland, Christopher P.:
Measuring Nuclear Mechanics with Atomic Force Microscopy.
In: Leake, Mark C. (Hrsg.): Chromosome Architecture : Methods and Protocols. - New York : Humana Press , 2022 . - S. 171-181
ISBN 978-1-0716-2221-6
DOI: https://doi.org/10.1007/978-1-0716-2221-6_13

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Abstract

Atomic force microscopy is an ideal tool to map topography and mechanical properties of materials on the micro- and nanoscale. Here, we describe its application to measure and analyze the mechanics, in particular the effective Young's elastic modulus E* of the mammalian nucleus in live cells. We present three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells which lack an established cytoskeleton, and purified nuclei to study their isolated response.