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Kämmer, Evelyn ; Olschewski, Konstanze ; Bocklitz, Thomas ; Rösch, Petra ; Weber, Karina ; Cialla, Dana ; Popp, Jürgen:
A new calibration concept for a reproducible quantitative detection based on SERS measurements in a microfluidic device demonstrated on the model analyte adenine.
In: Physical Chemistry Chemical Physics.
Bd. 16
(2014)
Heft 19
.
- S. 9056-9063.
ISSN 1463-9084
DOI: https://doi.org/10.1039/c3cp55312d
Abstract
This study demonstrates a new concept of calibrating surface enhanced Raman scattering (SERS) intensities without using additional substances as an internal standard and explores factors such as laser fluctuation and different Ag substrates, which affect the results of quantitative analyses based on SERS. To demonstrate the capabilities of the concept, the model analyte adenine has been chosen. A lab-on-a-chip device is applied for the measurements to guarantee consistent data recording. In order to simulate varied measuring conditions, two varying silver colloids (batch 1 and 2) are utilized as a SERS substrate and two different laser power levels (25 or 55 mW) are applied on the sample. A concentration gradient was generated which allows the use of the analyte itself for the correction of the resulting SERS spectra regarding intensity deviations caused by different ambient conditions. In doing so, a vast improvement in the quantification using SERS, especially in view of the comparability, reproducibility and repeatability, is demonstrated.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Mathematik, Physik und Informatik > Institut für Informatik > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 530 Physik |
| Eingestellt am: | 16 Mai 2023 12:34 |
| Letzte Änderung: | 16 Mai 2023 12:34 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/76329 |