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Kämmer, Evelyn ; Götz, Isabell ; Bocklitz, Thomas ; Stöckel, Stephan ; Dellith, Andrea ; Cialla-May, Dana ; Weber, Karina ; Zell, Roland ; Dellith, Jan ; Deckert, Volker ; Popp, Jürgen:
Single particle analysis of herpes simplex virus : Comparing the dimensions of one and the same virions via atomic force and scanning electron microscopy.
In: Analytical and Bioanalytical Chemistry.
Bd. 408
(2016)
Heft 15
.
- S. 4035-4041.
ISSN 1618-2650
DOI: https://doi.org/10.1007/s00216-016-9492-1
Abstract
Currently, two types of direct methods to characterize and identify single virions are available: electron microscopy (EM) and scanning probe techniques, especially atomic force microscopy (AFM). AFM in particular provides morphologic information even of the ultrastructure of viral specimens without the need to cultivate the virus and to invasively alter the sample prior to the measurements. Thus, AFM can play a critical role as a frontline method in diagnostic virology. Interestingly, varying morphological parameters for virions of the same type can be found in the literature, depending on whether AFM or EM was employed and according to the respective experimental conditions during the AFM measurements. Here, an inter-methodological proof of principle is presented, in which the same single virions of herpes simplex virus 1 were probed by AFM previously and after they were measured by scanning electron microscopy (SEM). Sophisticated chemometric analyses then allowed a calculation of morphological parameters of the ensemble of single virions and a comparison thereof. A distinct decrease in the virions’ dimensions was found during as well as after the SEM analyses and could be attributed to the sample preparation for the SEM measurements.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Mathematik, Physik und Informatik > Institut für Informatik > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie > Lehrstuhl Künstliche Intelligenz in der Mikroskopie und Spektroskopie - Univ.-Prof. Dr. Thomas Wilhelm Bocklitz |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 530 Physik |
| Eingestellt am: | 16 Mai 2023 12:31 |
| Letzte Änderung: | 16 Mai 2023 12:31 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/76330 |