Convenient detection of E. coli in Ringer's solution

Titelangaben

Schwarz, Martha ; Pahlow, Susanne ; Bocklitz, Thomas ; Steinbrücker, Carolin ; Cialla, Dana ; Weber, Karina ; Popp, Jürgen:
Convenient detection of E. coli in Ringer's solution.
In: Analyst. Bd. 138 (2013) Heft 20 . - S. 5866-5870.
ISSN 0003-2654
DOI: https://doi.org/10.1039/C3AN01240A

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Abstract

An easy and inexpensive detection method for DNA hybridization assays combining magnetic beads and enzymatically generated silver nanoparticles is introduced. The main advantage of this approach is the possibility to distinguish between positive and negative test results with the naked eye. In the case of complementary DNA sequences the sample will turn black within a few minutes, allowing readout without any hardware. In order to illustrate the applicability of the assay genomic DNA isolated from E. coli contaminated Ringer's solution was used for testing the sensitivity as well as specificity.