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Lateral spacing of integrin ligands influences cell spreading and focal adhesion assembly

Title data

Cavalcanti-Adam, Elisabetta Ada ; Micoulet, Alexandre ; Blümmel, Jacques ; Auernheimer, Jörg ; Kessler, Horst ; Spatz, Joachim P.:
Lateral spacing of integrin ligands influences cell spreading and focal adhesion assembly.
In: European Journal of Cell Biology. Vol. 85 (2006) Issue 3-4 . - pp. 219-224.
ISSN 1618-1298

Abstract in another language

Cell–extracellular matrix (cell–ECM) interactions mediated by integrin receptors are essential for providing positional and environmental information necessary for many cell functions, such as proliferation, differentiation and survival. In vitro studies on cell adhesion to randomly adsorbed molecules on substrates have been limited to sub-micrometer patches, thus preventing the detailed study of structural arrangement of integrins and their ligands. In this article, we illustrate the role of the distance between integrin ligands, namely the RGD (arginine–glycine–aspartate) sequence present in ECM proteins, in the control of cell adhesion. By using substrates, which carry cyclic RGD peptides arranged in highly defined nanopatterns, we investigated the dynamics of cell spreading and the molecular composition of adhesion sites in relation to a fixed spacing between the peptides on the surface. Our novel approach for in vitro studies on cell adhesion indicates that not only the composition, but also the spatial organization of the extracellular environment is important in regulating cell–ECM interactions.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 12 Jun 2023 14:00
Last Modified: 12 Jun 2023 14:00