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Bioactivity of xerogels as modulators of osteoclastogenesis mediated by connexin 43

Title data

Glenske, Kristina ; Wagner, Alena-Svenja ; Hanke, Thomas ; Cavalcanti-Adam, Elisabetta Ada ; Heinemann, Sascha ; Heinemann, Christiane ; Kruppke, Benjamin ; Arnhold, Stefan ; Moritz, Andreas ; Schwab, Elisabeth H. ; Worch, Hartmut ; Wenisch, Sabine:
Bioactivity of xerogels as modulators of osteoclastogenesis mediated by connexin 43.
In: Biomaterials. Vol. 35 (2014) Issue 5 . - pp. 1487-1495.
ISSN 0142-9612

Abstract in another language

In order to investigate the effects of different degrees of bioactivity of xerogels on connexin 43 (cx43) signaling of osteoclasts a cell culture approach was developed. Cells isolated from peripheral blood mononuclear cells were cultured in combination with the xerogels and were harvested for further investigations on day 1, day 5, and day 10. By means of quantitative PCR increased cx43 mRNA levels and coincident decreasing mRNA levels of the calcium sensing receptor, TRAP, and Cathepsin K were detected with increasing bioactivity of the xerogel samples. Additionally, osteoclasts cultured on tissue culture plates were used to perform principle investigations on cell differentiation by means of transmission electron microscopy, life cell imaging, and immunofluorescence, and the results demonstrated that cx43-signaling could be attributed to migration and fusion of osteoclast precursors. Therefore, the positive correlation of cx43 expression with high xerogel bioactivity was caused by proceeding differentiation of the osteoclasts. Finally, the presently observed pattern of cx43 signaling refers to strong effects regarding bioactivity on cx43-associated cell differentiation of osteoclasts influenced by extracellular calcium ions.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Bioactivity; Calcium; Osteoclast; Xerogel
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 09 Jun 2023 10:48
Last Modified: 09 Jun 2023 10:48