bei Google ScholarTitelangaben
Glenske, Kristina ; Wagner, Alena-Svenja ; Hanke, Thomas ; Cavalcanti-Adam, Elisabetta Ada ; Heinemann, Sascha ; Heinemann, Christiane ; Kruppke, Benjamin ; Arnhold, Stefan ; Moritz, Andreas ; Schwab, Elisabeth H. ; Worch, Hartmut ; Wenisch, Sabine:
Bioactivity of xerogels as modulators of osteoclastogenesis mediated by connexin 43.
In: Biomaterials.
Bd. 35
(2014)
Heft 5
.
- S. 1487-1495.
ISSN 0142-9612
DOI: https://doi.org/10.1016/j.biomaterials.2013.11.002
Abstract
In order to investigate the effects of different degrees of bioactivity of xerogels on connexin 43 (cx43) signaling of osteoclasts a cell culture approach was developed. Cells isolated from peripheral blood mononuclear cells were cultured in combination with the xerogels and were harvested for further investigations on day 1, day 5, and day 10. By means of quantitative PCR increased cx43 mRNA levels and coincident decreasing mRNA levels of the calcium sensing receptor, TRAP, and Cathepsin K were detected with increasing bioactivity of the xerogel samples. Additionally, osteoclasts cultured on tissue culture plates were used to perform principle investigations on cell differentiation by means of transmission electron microscopy, life cell imaging, and immunofluorescence, and the results demonstrated that cx43-signaling could be attributed to migration and fusion of osteoclast precursors. Therefore, the positive correlation of cx43 expression with high xerogel bioactivity was caused by proceeding differentiation of the osteoclasts. Finally, the presently observed pattern of cx43 signaling refers to strong effects regarding bioactivity on cx43-associated cell differentiation of osteoclasts influenced by extracellular calcium ions.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | Bioactivity; Calcium; Osteoclast; Xerogel |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik > Lehrstuhl Zelluläre Biomechanik - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 09 Jun 2023 10:48 |
| Letzte Änderung: | 09 Jun 2023 10:48 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81182 |