Literature by the same author
plus at Google Scholar

Bibliografische Daten exportieren

Enhanced Biological Activity of BMP‐2 Bound to Surface‐Grafted Heparan Sulfate

Title data

Migliorini, Elisa ; Horn, Patrick ; Haraszti, Tamás ; Wegner, Seraphine V. ; Hiepen, Christian ; Knaus, Petra ; Richter, Ralf P. ; Cavalcanti-Adam, Elisabetta Ada:
Enhanced Biological Activity of BMP‐2 Bound to Surface‐Grafted Heparan Sulfate.
In: Advanced Biosystems. Vol. 1 (2017) Issue 4 . - 1600041.
ISSN 2366-7478

Abstract in another language

Over the last decade, there has been a growing interest in the development of new materials to improve bone morphogenetic protein-2 (BMP-2) delivery for tissue regeneration. This study reports the development and application of model surfaces that present BMP-2 via heparan sulfate (HS), a ubiquitous component of the extracellular matrix (ECM). On these surfaces, HS is grafted by its reducing end, to mimic the natural arrangement of HS proteoglycans in the ECM. The binding of each component on these biomimetic surfaces is highly controlled, in terms of stoichiometry of molecules and BMP-2/grafted-HS affinity, as determined by surface-sensitive techniques. For comparison, this study also uses surfaces presenting immobilized BMP-2 alone. Functional validations of the surfaces are performed using a murine myoblast cell line (C2C12) and primary human mesenchymal stromal cells. In both cell types, HS-bound BMP-2 and surface-immobilized BMP-2 significantly prolong SMAD 1/5 phosphorylation, compared to BMP-2 added to the culture media. Moreover, HS-bound BMP-2 enhances p-SMAD 1/5 levels in C2C12 cells and reduces noggin antagonistic activity. Thus, grafted HS positively affects BMP-2 cellular activity. This innovative surface design, which mimics natural interactions of growth factors with ECM components, constitutes a promising candidate for future regenerative medicine applications.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 09 Jun 2023 07:05
Last Modified: 09 Jun 2023 07:05