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Surface Co-presentation of BMP-2 and integrin selective ligands at the nanoscale favors α₅β₁ integrin-mediated adhesion

Title data

Posa, Francesca ; Baha-Schwab, Elisabeth H. ; Wei, Qiang ; Di Benedetto, Adriana ; Neubauer, Stefanie ; Reichart, Florian ; Kessler, Horst ; Spatz, Joachim P. ; Albiges-Rizo, Corinne ; Mori, Giorgio ; Cavalcanti-Adam, Elisabetta Ada:
Surface Co-presentation of BMP-2 and integrin selective ligands at the nanoscale favors α₅β₁ integrin-mediated adhesion.
In: Biomaterials. Vol. 267 (2021) . - 120484.
ISSN 0142-9612

Abstract in another language

Here we present the use of surface nanopatterning of covalently immobilized BMP-2 and integrin selective ligands to determine the specificity of their interactions in regulating cell adhesion and focal adhesion assembly. Gold nanoparticle arrays carrying single BMP-2 dimers are prepared by block-copolymer micellar nanolithography and azide-functionalized integrin ligands (cyclic-RGD peptides or α5β1 integrin peptidomimetics) are immobilized on the surrounding polyethylene glycol alkyne by click chemistry. Compared to BMP-2 added to the media, surface immobilized BMP-2 (iBMP-2) favors the spatial segregation of adhesion clusters and enhances focal adhesion (FA) size in cells adhering to α5β1 integrin selective ligands. Moreover, iBMP-2 copresented with α5β1 integrin ligands induces the recruitment of αvβ3 integrins in FAs. When copresented with RGD, iBMP-2 induces the assembly of a higher number of FAs, which are not affected by α5β1 integrin blocking. Our dual-functionalized platforms offer the possibility to study the crosstalk between integrins and BMP receptors, and more in general they could be used to address the spatial regulation of growth factors and adhesion receptors crosstalk on biomimetic surfaces.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: BMP-2; Integrins; Focal adhesions; Surface functionalization; Click chemistry
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 07 Jun 2023 06:30
Last Modified: 07 Jun 2023 06:30