at Google ScholarTitle data
Migliorini, Elisa ; Cavalcanti-Adam, Elisabetta Ada ; Uva, Antonio Emmanuele ; Fiorentino, Michele ; Gattullo, Michele ; Manghisi, Vito Modesto ; Vaiani, Lorenzo ; Boccaccio, Antonio:
Nanoindentation of mesenchymal stem cells using atomic force microscopy : effect of adhesive cell-substrate structures.
In: Nanotechnology.
Vol. 32
(2021)
Issue 21
.
- 215706.
ISSN 1361-6528
DOI: https://doi.org/10.1088/1361-6528/abe748
Abstract in another language
The procedure commonly adopted to characterize cell materials using atomic force microscopy neglects the stress state induced in the cell by the adhesion structures that anchor it to the substrate. In several studies, the cell is considered as made from a single material and no specific information is provided regarding the mechanical properties of subcellular components. Here we present an optimization algorithm to determine separately the material properties of subcellular components of mesenchymal stem cells subjected to nanoindentation measurements. We assess how these properties change if the adhesion structures at the cell-substrate interface are considered or not in the algorithm. In particular, among the adhesion structures, the focal adhesions and the stress fibers were simulated. We found that neglecting the adhesion structures leads to underestimate the cell mechanical properties thus making errors up to 15%. This result leads us to conclude that the action of adhesion structures should be taken into account in nanoindentation measurements especially for cells that include a large number of adhesions to the substrate.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics |
| Result of work at the UBT: | No |
| DDC Subjects: | 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 07 Jun 2023 06:30 |
| Last Modified: | 07 Jun 2023 06:30 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81228 |