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Schoenit, Andreas ; Lo Giudice, Cristina ; Hahnen, Nina ; Ollech, Dirk ; Jahnke, Kevin ; Göpfrich, Kerstin ; Cavalcanti-Adam, Elisabetta Ada:
Tuning Epithelial Cell–Cell Adhesion and Collective Dynamics with Functional DNA-E-Cadherin Hybrid Linkers.
In: Nano Letters.
Bd. 22
(2022)
Heft 1
.
- S. 302-310.
ISSN 1530-6992
DOI: https://doi.org/10.1021/acs.nanolett.1c03780
Abstract
The binding strength between epithelial cells is crucial for tissue integrity, signal transduction and collective cell dynamics. However, there is no experimental approach to precisely modulate cell–cell adhesion strength at the cellular and molecular level. Here, we establish DNA nanotechnology as a tool to control cell–cell adhesion of epithelial cells. We designed a DNA-E-cadherin hybrid system consisting of complementary DNA strands covalently bound to a truncated E-cadherin with a modified extracellular domain. DNA sequence design allows to tune the DNA-E-cadherin hybrid molecular binding strength, while retaining its cytosolic interactions and downstream signaling capabilities. The DNA-E-cadherin hybrid facilitates strong and reversible cell–cell adhesion in E-cadherin deficient cells by forming mechanotransducive adherens junctions. We assess the direct influence of cell–cell adhesion strength on intracellular signaling and collective cell dynamics. This highlights the scope of DNA nanotechnology as a precision technology to study and engineer cell collectives.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | cell−cell adhesion strength; E-cadherin; DNA nanotechnology; adherens junction; epithelial cells; collective migration; DNA−protein hybrid; mechanotransduction |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik > Lehrstuhl Zelluläre Biomechanik - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Zelluläre Biomechanik |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 07 Jun 2023 06:26 |
| Letzte Änderung: | 07 Jun 2023 06:26 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/81232 |