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Tuning Epithelial Cell–Cell Adhesion and Collective Dynamics with Functional DNA-E-Cadherin Hybrid Linkers

Title data

Schoenit, Andreas ; Lo Giudice, Cristina ; Hahnen, Nina ; Ollech, Dirk ; Jahnke, Kevin ; Göpfrich, Kerstin ; Cavalcanti-Adam, Elisabetta Ada:
Tuning Epithelial Cell–Cell Adhesion and Collective Dynamics with Functional DNA-E-Cadherin Hybrid Linkers.
In: Nano Letters. Vol. 22 (2022) Issue 1 . - pp. 302-310.
ISSN 1530-6992
DOI: https://doi.org/10.1021/acs.nanolett.1c03780

Abstract in another language

The binding strength between epithelial cells is crucial for tissue integrity, signal transduction and collective cell dynamics. However, there is no experimental approach to precisely modulate cell–cell adhesion strength at the cellular and molecular level. Here, we establish DNA nanotechnology as a tool to control cell–cell adhesion of epithelial cells. We designed a DNA-E-cadherin hybrid system consisting of complementary DNA strands covalently bound to a truncated E-cadherin with a modified extracellular domain. DNA sequence design allows to tune the DNA-E-cadherin hybrid molecular binding strength, while retaining its cytosolic interactions and downstream signaling capabilities. The DNA-E-cadherin hybrid facilitates strong and reversible cell–cell adhesion in E-cadherin deficient cells by forming mechanotransducive adherens junctions. We assess the direct influence of cell–cell adhesion strength on intracellular signaling and collective cell dynamics. This highlights the scope of DNA nanotechnology as a precision technology to study and engineer cell collectives.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: cell−cell adhesion strength; E-cadherin; DNA nanotechnology; adherens junction; epithelial cells; collective migration; DNA−protein hybrid; mechanotransduction
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 07 Jun 2023 06:26
Last Modified: 07 Jun 2023 06:26
URI: https://eref.uni-bayreuth.de/id/eprint/81232