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Integrin αIIbβ₃ activation and clustering in minimal synthetic cells

Title data

Benk, Lucia T. ; Benk, Amelie S. ; Lira, Rafael B. ; Cavalcanti-Adam, Elisabetta Ada ; Dimova, Rumiana ; Lipowsky, Reinhard ; Geiger, Benjamin ; Spatz, Joachim P.:
Integrin αIIbβ₃ activation and clustering in minimal synthetic cells.
In: Advanced NanoBiomed Research. Vol. 2 (2022) Issue 4 . - 2100094.
ISSN 2699-9307

Abstract in another language

Platelet adhesion and activation are mediated by integrin αIIbβ3 clustering, which is crucial for the hemostatic function of platelets. In an activated state, integrins provide the connection between the extracellular matrix and the actin cytoskeleton through a variety of cytoplasmic proteins, such as talin. Here, droplet-based microfluidics is applied to generate cell-sized giant unilamellar vesicles (GUVs) with a defined molecular composition to quantify the adhesion of integrin αIIbβ3-containing protocells in relation to the number of integrin–talin head domain (THD) complexes. Furthermore, it is shown that THD induces integrin clustering in protocells adhering to fibrinogen. The formation of this molecular link, which has, so far, only been observed in vivo, is an essential step in synthetic cell design to recapitulate integrin-mediated bidirectional signaling across the membrane. These results pave the way for further quantitative investigations of protein–protein interactions between integrins and associated proteins and their assembly within such defined, but complex, synthetic cells. An essential future step to mimic the complex interaction between cells and their environment will be to combine synthetic approaches with peptide chemistry to guide the molecular mechanisms involved in integrin binding and activation.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics
Result of work at the UBT: No
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 07 Jun 2023 06:28
Last Modified: 07 Jun 2023 06:28