Colloidally Stable and Surfactant-Free Protein-Coated Gold Nanorods in Biological Media

Titelangaben

Tebbe, Moritz ; Kuttner, Christian ; Männel, Max ; Fery, Andreas ; Chanana, Munish:
Colloidally Stable and Surfactant-Free Protein-Coated Gold Nanorods in Biological Media.
In: ACS Applied Materials & Interfaces. Bd. 7 (2015) Heft 10 . - S. 5984-5991.
ISSN 1944-8252
DOI: https://doi.org/10.1021/acsami.5b00335

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Abstract

In this work, we investigate the ligand exchange of cetyltrimethylammonium bromide (CTAB) with bovine serum albumine (BSA) for gold nanorods. We demonstrate by surface-enhanced Raman scattering (SERS) measurements, that CTAB, which is used as a shape-directing agent in the particle synthesis, is completely removed from solution and particle surface. Thus the protein-coated nanorods are suitable for bioapplications, where cationic surfactants have to be avoided. At the same time, the colloidal stability of the system is significantly increased, as evidenced by spectroscopic investigation of the particles longitudinal surface plasmon resonance, which is sensitive towards aggregation. Particles are stable at very high concentrations (cAu 20 mg/mL) in biological media such as phosphate buffer saline (PBS) or Dulbecco’s Modified Eagle’s Medium (DMEM) and over a large pH-range (2-12). Particles can even be freeze-dried (lyophilized) and redispersed. The protocol was applied to gold nanoparticles with a large range of aspect ratios and sizes with main absorption frequencies covering the visible and the near infrared (NIR) spectral range from 600 to 1100 nm. Thus, these colloidally stable and surfactant-free protein-coated nanoparticles are of great interest for various plasmonic and biomedical applications.