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Design of Recombinant Spider Silk Proteins for Cell Type Specific Binding

Title data

Trossmann, Vanessa T. ; Scheibel, Thomas:
Design of Recombinant Spider Silk Proteins for Cell Type Specific Binding.
In: Advanced Healthcare Materials. Vol. 12 (2023) Issue 9 . - 2202660.
ISSN 2192-2659

Official URL: Volltext

Project information

Project financing: Deutsche Forschungsgemeinschaft
Elitenetzwerk Bayern

Abstract in another language

Abstract Cytophilic (cell-adhesive) materials are very important for tissue engineering and regenerative medicine. However, for engineering hierarchically organized tissue structures comprising different cell types, cell-specific attachment and guidance are decisive. In this context, materials made of recombinant spider silk proteins are promising scaffolds, since they exhibit high biocompatibility, biodegradability, and the underlying proteins can be genetically functionalized. Here, previously established spider silk variants based on the engineered Araneus diadematus fibroin 4 (eADF4(C16)) are genetically modified with cell adhesive peptide sequences from extracellular matrix proteins, including IKVAV, YIGSR, QHREDGS, and KGD. Interestingly, eADF4(C16)-KGD as one of 18 tested variants is cell-selective for C2C12 mouse myoblasts, one out of 11 tested cell lines. Co-culturing with B50 rat neuronal cells confirms the cell-specificity of eADF4(C16)-KGD material surfaces for C2C12 mouse myoblast adhesion.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: bioactive peptides; bioselectivity; cell co-culture; cytophilic surfaces; gradient materials
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 15 Jul 2023 21:12
Last Modified: 17 Jul 2023 09:45