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A promising method for fast identification of microplastic particles in environmental samples : A pilot study using fluorescence lifetime imaging microscopy

Title data

Wohlschläger, Maximilian ; Versen, Martin ; Löder, Martin G. J. ; Laforsch, Christian:
A promising method for fast identification of microplastic particles in environmental samples : A pilot study using fluorescence lifetime imaging microscopy.
In: Heliyon. Vol. 10 (2024) Issue 3 . - e25133.
ISSN 2405-8440
DOI: https://doi.org/10.1016/j.heliyon.2024.e25133

Project information

Project title:
Project's official title
Project's id
SFB 1357 Mikroplastik
391977956

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Microplastic pollution of the environment has been extensively studied, with recent studies focusing on the prevalence of microplastics in the environment and their effects on various organisms. Identification methods that simplify the extraction and analysis process to the point where the extraction can be omitted are being investigated, thus enabling the direct identification of microplastic particles. Currently, microplastic samples from environmental matrices can only be identified using time-consuming extraction, sample processing, and analytical methods. Various spectroscopic methods are currently employed, such as micro Fourier-transform infrared, attenuated total reflectance, and micro Raman spectroscopy. However, microplastics in environmental matrices cannot be directly identified using these spectroscopic methods. Investigations using frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) to identify and differentiate plastics from environmental materials have yielded promising results for directly identifying microplastics in an environmental matrix. Herein, two artificially prepared environmental matrices that included natural soil, grass, wood, and high-density polyethylene were investigated using FD-FLIM. Our first results showed that we successfully identified one plastic type in the two artificially prepared matrices using FD-FLIM. However, further research must be conducted to improve the FD-FLIM method and explore its limitations for directly identifying microplastics in environmental samples.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Microplastics; Material identification; Environmental science; Fluorescence lifetime; FD-FLIM; Fluorescence microscopy
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology I
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology I > Chair Animal Ecology I - Univ.-Prof. Dr. Christian Laforsch
Research Institutions > Central research institutes > Bayreuth Center of Ecology and Environmental Research- BayCEER
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 530 Physics
500 Science > 540 Chemistry
500 Science > 550 Earth sciences, geology
500 Science > 570 Life sciences, biology
Date Deposited: 06 Feb 2024 09:26
Last Modified: 06 Feb 2024 09:26
URI: https://eref.uni-bayreuth.de/id/eprint/88465