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An RNA Motif That Enables Optozyme Control and Light-Dependent Gene Expression in Bacteria and Mammalian Cells

Title data

Pietruschka, Georg ; Ranzani, Américo Tavares ; Weber, Anna ; Patwari, Tejal ; Pilsl, Sebastia ; Renzl, Christian ; Otte, David M. ; Pyka, Daniel ; Möglich, Andreas ; Mayer, Günter:
An RNA Motif That Enables Optozyme Control and Light-Dependent Gene Expression in Bacteria and Mammalian Cells.
In: Advanced Science. Vol. 11 (2024) Issue 12 . - 2304519.
ISSN 2198-3844

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

The regulation of gene expression by light enables the versatile, spatiotemporal manipulation of biological function in bacterial and mammalian cells. Optoribogenetics extends this principle by molecular RNA devices acting on the RNA level whose functions are controlled by the photoinduced interaction of a light-oxygen-voltage photoreceptor with cognate RNA aptamers. Here light-responsive ribozymes, denoted optozymes, which undergo light-dependent self-cleavage and thereby control gene expression are described. This approach transcends existing aptamer-ribozyme chimera strategies that predominantly rely on aptamers binding to small molecules. The optozyme method thus stands to enable the graded, non-invasive, and spatiotemporally resolved control of gene expression. Optozymes are found efficient in bacteria and mammalian cells and usher in hitherto inaccessible optoribogenetic modalities with broad applicability in synthetic and systems biology.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: synthetic biology; optogenetics; aptamers; SELEX; ribozymes; LOV-domains
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Andreas Möglich
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 23 Apr 2024 08:24
Last Modified: 23 Apr 2024 08:24